modified double skeletal staining protocols with alizarin red and alcian blue in laboratory animals
نویسندگان
چکیده
abstract purpose: skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.this article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. materials and methods: the important notes about skeletal staining were fixing by ethanol and clearing by potassium hydroxide in embryonic and newborn samples. in adults it was fixing by neutral formalin buffer, then ethanol after washing the samples in ddh2o, and clearing the samples by trypsin and potassium hydroxide in separated stages. the amount of colors used for cartilages and bones was differentin animals with different ages for a good stained sample. results: the mentioned procedures resulted in completely clear stained samples whose skeletal parts, i.e. cartilages and bones,were stained blue and red, respectively. conclusion: although most reviewed investigations have used the same protocol in different laboratory animals with different ages, the used materials and also their concentrations for skeletal staining procedures differ in embryos and adults.
منابع مشابه
Modified double skeletal staining protocols with Alizarin red and Alcian blue in laboratory animals
Abstract Purpose: Skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.This article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. Materials and Methods: The important notes about skeletal staining were fixing by ethan...
متن کاملTwo Separated Protocols with the Most Important Comments for Skeletal Staining in Embryonic and Adulthood Period in Laboratory Animals
Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...
متن کاملtwo separated protocols with the most important comments for skeletal staining in embryonic and adulthood period in laboratory animals
introduction: skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. the aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. methods: in this paper, s...
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عنوان ژورنال:
annals of military and health science researchجلد ۱۳، شماره ۲، صفحات ۷۶-۸۱
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